Embryo Rescue Technique in Buckwheat Fagopyrum esculentum Moench.

ABSTRACT

Culture of immature buckwheat embryos is an excellent explant for tissue culture and regeneration in vitro, as well as a method of embryo rescue in case of fruit abortion especially in interspecific crosses. Here, we describe the method of immature embryo rescue using a differentiated approach to different age groups. The method involves the development of embryos along the path of direct embryogenesis bypassing the callus stage.