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The synthesis of broccoli sprout extract-loaded silk fibroin nanoparticles as efficient drug delivery vehicles: development and characterization.
Ghanbari Hassan Kiadeh, Saeed; Rahaiee, Somayeh; Azizi, Hossein; Govahi, Mostafa.
Affiliation
  • Ghanbari Hassan Kiadeh S; Department of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.
  • Rahaiee S; Department of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.
  • Azizi H; Department of Nano Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.
  • Govahi M; Department of Nano Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran.
Pharm Dev Technol ; 29(4): 359-370, 2024 Apr.
Article in En | MEDLINE | ID: mdl-38546461
ABSTRACT
Targeted drug delivery of biological molecules using the development of biocompatible, non-toxic and biodegradable nanocarriers can be a promising method for cancer therapy. In this study, silk fibroin protein nanoparticles (SFPNPs) were synthesized as a targeted delivery system for sulforaphane-rich broccoli sprout extract (BSE). The BSE-loaded SFPNPs were conjugated with polyethylene glycol and folic acid, and then their physicochemical properties were characterized via UV-Vis, XRD, FTIR, DLS, FE-SEM and EDX analyses. In vitro, the release profile, antioxidant and anticancer activities of NPs were also studied. The FE-SEM and DLS analyses indicated stable NPs with an average size of 88.5 nm and high zeta potential (-32 mV). The sulforaphane release profile from NPs was pH-dependent, with the maximum release value (70%) observed in simulated intestinal fluid (pH = 7.4). Encapsulation of BSE also decreased the release rate of sulforaphane from the capsules compared to free BSE. In vitro cytotoxicity of BSE and NPs on breast cancer cell lines (MCF-7) was concentration-dependent, and the IC50 for BSE and NPs were 54 and 210 µg ml-1, respectively. Moreover, the NPs demonstrated no appreciable cytotoxicity in normal mouse fibroblast (L929) cell lines. These results indicated that biocompatible NPs synthesized as controlled and long-term targeted drug delivery systems can be a potential candidate for breast cancer therapy.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sulfoxides / Brassica / Plant Extracts / Isothiocyanates / Nanoparticles / Fibroins Limits: Animals / Humans Language: En Journal: Pharm Dev Technol / Pharm. dev. technol / Pharmaceutical development and technology Journal subject: FARMACIA Year: 2024 Document type: Article Affiliation country: Irán Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sulfoxides / Brassica / Plant Extracts / Isothiocyanates / Nanoparticles / Fibroins Limits: Animals / Humans Language: En Journal: Pharm Dev Technol / Pharm. dev. technol / Pharmaceutical development and technology Journal subject: FARMACIA Year: 2024 Document type: Article Affiliation country: Irán Country of publication: Reino Unido