Serum Prestin After Ototoxin Exposure Is Not Dependent on Outer Hair Cell Loss.
Otol Neurotol
; 45(5): 495-501, 2024 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-38561601
ABSTRACT
HYPOTHESIS:
Cyclodextrin (CDX)-induced serum prestin burst is not dependent on outer hair cell (OHC) loss.BACKGROUND:
Serum prestin has been proposed as a biomarker for ototoxicity. We recently used an automated Western approach to quantify serum prestin changes in a newly introduced model of CDX ototoxicity. To gain insights into prestin as a biomarker, here we further characterize serum prestin in the CDX model.METHODS:
Guinea pigs were treated with 750, 3,000, or 4,000 mg/kg CDX, and serum samples were obtained through up to 15 weeks after exposure. Serum prestin levels were quantified using automated Western, and hair cell counts were obtained.RESULTS:
All three doses induced an N -glycosylated ~134-kDa prestin burst; however, only the 3,000 and 4,000 mg/kg resulted in robust OHC loss. Prestin levels returned to baseline where they remained up to 15 weeks in the absence of OHCs.CONCLUSION:
The ~134-kDa prestin burst induced after CDX administration is N -glycosylated, representing a posttranslational modification of prestin. Serum prestin seems to be a promising biomarker when using therapeutics with ototoxic properties because it is not dependent on OHC loss as a necessary event, thus affording the opportunity for early detection and intervention.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hair Cells, Auditory, Outer
Limits:
Animals
Language:
En
Journal:
Otol Neurotol
Journal subject:
NEUROLOGIA
/
OTORRINOLARINGOLOGIA
Year:
2024
Document type:
Article
Country of publication:
Estados Unidos