Development of an on-site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues.
Pest Manag Sci
; 80(8): 4069-4073, 2024 Aug.
Article
in En
| MEDLINE
| ID: mdl-38563512
ABSTRACT
BACKGROUND:
The tomato leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera Gelechiidae), is a destructive invasive pest that originated in South America and has spread within China since 2017. A rapid method for on-site identification of P. absoluta is urgently needed for interception of this pest across China.RESULTS:
We developed a loop-mediated isothermal amplification (LAMP) technique to differentiate P. absoluta from Liriomyza sativae, Chromatomyia horticola, and Phthorimaea operculella using extracted genomic DNA, which was then refined to create an on-site LAMP diagnostic method that can be performed under field conditions without the need for laboratory equipment.CONCLUSION:
In the present research, we developed an on-site diagnostic method for rapid differentiation of P. absoluta from other insects with similar morphology or damage characteristics in China. © 2024 Society of Chemical Industry.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nucleic Acid Amplification Techniques
/
Introduced Species
/
Moths
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Pest Manag Sci
Journal subject:
TOXICOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Reino Unido