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Noninvasive In Planta Live Measurements of H2O2 and Glutathione Redox Potential with Fluorescent roGFPs-Based Sensors.
Buratti, Stefano; Grenzi, Matteo; Tortora, Giorgia; Nastasi, Sara Paola; Dell'Aglio, Elisa; Bassi, Andrea; Costa, Alex.
Affiliation
  • Buratti S; Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
  • Grenzi M; Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
  • Tortora G; Department of Physics, Politecnico di Milano, Milan, Italy.
  • Nastasi SP; Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
  • Dell'Aglio E; Department of Biosciences, Università degli Studi di Milano, Milan, Italy.
  • Bassi A; Department of Physics, Politecnico di Milano, Milan, Italy.
  • Costa A; Department of Biosciences, Università degli Studi di Milano, Milan, Italy. alex.costa@unimi.it.
Methods Mol Biol ; 2798: 45-64, 2024.
Article in En | MEDLINE | ID: mdl-38587735
ABSTRACT
In this protocol, we present a noninvasive in planta bioimaging technique for the analysis of hydrogen peroxide (H2O2) and glutathione redox potential in adult Arabidopsis thaliana plants. The technique is based on the use of stereo fluorescence microscopy to image A. thaliana plants expressing the two genetically encoded fluorescent sensors roGFP2-Orp1 and Grx1-roGFP2. We provide a detailed step-by-step protocol for performing low magnification imaging with mature plants grown in soil or hydroponic systems. This protocol aims to serve the scientific community by providing an accessible approach to noninvasive in planta bioimaging and data analysis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / Hydrogen Peroxide Limits: Adult / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Italia
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / Hydrogen Peroxide Limits: Adult / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: Italia