Your browser doesn't support javascript.
loading
Enhancing APE1 detection through apurinic/apyrimidinic site inhibition of DNA polymerase: an innovative, highly sensitive approach.
Liu, Zhijun; Yan, Bei; Liu, Huan; Liu, Xiao; Xiao, Xianjin; Ming, Zhihao.
Affiliation
  • Liu Z; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. xiaoxianjin@hust.edu.cn.
  • Yan B; Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, China.
  • Liu H; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. xiaoxianjin@hust.edu.cn.
  • Liu X; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. xiaoxianjin@hust.edu.cn.
  • Xiao X; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. xiaoxianjin@hust.edu.cn.
  • Ming Z; Department of Urology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410006, China. mingzhihao@hnca.org.cn.
Chem Commun (Camb) ; 60(35): 4695-4698, 2024 Apr 25.
Article in En | MEDLINE | ID: mdl-38592754
ABSTRACT
This study presents an innovative method for the highly sensitive detection of apurinic/apyrimidinic endonuclease 1 (APE1), a crucial biomarker and target for cancer diagnosis and treatment. The method is predicated on our discovery that the apurinic or apyrimidinic site (AP site) can inhibit the activity of Taq DNA polymerase. Subsequent experiments further led to the development of a new amplification method based on the digestion activity of Lambda exonuclease. This approach showed potential to detect trace amounts of APE1 in biological samples with high sensitivity.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA-(Apurinic or Apyrimidinic Site) Lyase Limits: Humans Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2024 Document type: Article Affiliation country: China
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA-(Apurinic or Apyrimidinic Site) Lyase Limits: Humans Language: En Journal: Chem Commun (Camb) Journal subject: QUIMICA Year: 2024 Document type: Article Affiliation country: China