Generation and characterization of the iPS cell line (SYSUSHi001-A) derived from the peripheral blood mononuclear cells (PBMCs) of a 33-year-old patient with acute myeloid leukemia (AML).
Stem Cell Res
; 77: 103360, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38608356
ABSTRACT
We successfully developed an induced pluripotent stem cell (iPSC) line, SYSUSHi001-A, from the peripheral blood mononuclear cells (PBMC) of a patient with Acute Myeloid Leukemia, harboring two genetic mutations (XPO1 c.591-4_591-3dupTT; PALB2 c.3296C > T; p.T1099M). This iPSC line was facilitated through the use of episomal plasmids encoding OCT4, SOX2, KLF4, L-MYC, and human miR-302. The SYSUSHi001-A iPSC line exhibited characteristic embryonic stem cell-like morphology, maintained the XPO1 and PALB2 mutations, expressed key pluripotency markers, preserved a normal karyotype (46, XY), and demonstrated the ability to differentiate into cells from all three germ layers in vitro.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Leukocytes, Mononuclear
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Leukemia, Myeloid, Acute
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Induced Pluripotent Stem Cells
/
Kruppel-Like Factor 4
Limits:
Adult
/
Humans
/
Male
Language:
En
Journal:
Stem Cell Res
Year:
2024
Document type:
Article
Affiliation country:
China