Photocatalytic manipulation of Ca2+ signaling for regulating cellular and animal behaviors via MOF-enabled H2O2 generation.
Sci Adv
; 10(16): eadl0263, 2024 Apr 19.
Article
in En
| MEDLINE
| ID: mdl-38640246
ABSTRACT
The in situ generation of H2O2 in cells in response to external stimulation has exceptional advantages in modulating intracellular Ca2+ dynamics, including high controllability and biological safety, but has been rarely explored. Here, we develop photocatalyst-based metal-organic frameworks (DCSA-MOFs) to modulate Ca2+ responses in cells, multicellular spheroids, and organs. By virtue of the efficient photocatalytic oxygen reduction to H2O2 without sacrificial agents, photoexcited DCSA-MOFs can rapidly trigger Ca2+ outflow from the endoplasmic reticulum with single-cell precision in a repeatable and controllable manner, enabling the propagation of intercellular Ca2+ waves (ICW) over long distances in two-dimensional and three-dimensional cell cultures. After photoexcitation, ICWs induced by DCSA-MOFs can activate neural activities in the optical tectum of tadpoles and thighs of spinal frogs, eliciting the corresponding motor behaviors. Our study offers a versatile optical nongenetic modulation technique that enables remote, repeatable, and controlled manipulation of cellular and animal behaviors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Calcium Signaling
/
Hydrogen Peroxide
Limits:
Animals
Language:
En
Journal:
Sci Adv
/
Sci. Adv
/
Science advances
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Estados Unidos