Genome-Driven Discovery of Hygrocins in Streptomyces rapamycinicus.
J Nat Prod
; 87(5): 1321-1329, 2024 May 24.
Article
in En
| MEDLINE
| ID: mdl-38647518
ABSTRACT
Ansamycins, represented by the antituberculosis drug rifamycin, are an important family of natural products. To obtain new ansamycins, Streptomyces rapamycinicus IMET 43975 harboring an ansamycin biosynthetic gene cluster was fermented in a 50 L scale, and subsequent purification work led to the isolation of five known and four new analogues, where hygrocin W (2) belongs to benzoquinonoid ansamycins, and the other three hygrocins, hygrocins X-Z (6-8), are new seco-hygrocins. The structures of ansamycins (1-8) were determined by the analysis of spectroscopic (1D/2D NMR and ECD) and MS spectrometric data. The Baeyer-Villiger enzyme which catalyzed the ester formation in the ansa-ring was confirmed through in vivo CRISPR base editing. The discovery of these compounds further enriches the structural diversity of ansamycins.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Streptomyces
Language:
En
Journal:
J Nat Prod
Year:
2024
Document type:
Article
Affiliation country:
Dinamarca
Country of publication:
Estados Unidos