An injectable active hydrogel based on BMSC-derived extracellular matrix for cartilage regeneration enhancement.
Biomater Adv
; 160: 213857, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38657287
ABSTRACT
Articular cartilage injury impairs joint function and necessitates orthopedic intervention to restore the structure and function of the cartilage. Extracellular matrix (ECM) scaffolds derived from bone marrow mesenchymal stem cells (BMSCs) can effectively promote cell adhesion, proliferation, and chondrogenesis. However, pre-shaped ECM scaffolds have limited applicability due to their poor fit with the irregular surface of most articular cartilage defects. In this study, we fabricated an injectable active ECM hydrogel from autologous BMSCs-derived ECM by freeze-drying, liquid nitrogen milling, and enzymatic digestion. Moreover, our in vitro and in vivo results demonstrated that the prepared hydrogel enhanced chondrocyte adhesion and proliferation, chondrogenesis, cartilage regeneration, and integration with host tissue, respectively. These findings indicate that active ECM components can provide trophic support for cell proliferation and differentiation, restoring the structure and function of damaged cartilage.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Regeneration
/
Cartilage, Articular
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Chondrocytes
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Hydrogels
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Chondrogenesis
/
Tissue Engineering
/
Extracellular Matrix
/
Tissue Scaffolds
/
Mesenchymal Stem Cells
Limits:
Animals
/
Humans
Language:
En
Journal:
Biomater Adv
Year:
2024
Document type:
Article
Country of publication:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS