A Phenanthrenequinone-Based Ratiometric Fluorescent Probe for Rapid Detection of Peroxynitrite with Imaging in Osteoblast Precursor Cells.
Chem Res Toxicol
; 37(5): 771-778, 2024 May 20.
Article
in En
| MEDLINE
| ID: mdl-38658839
ABSTRACT
In the current situation, peroxynitrite (ONOO-) is drawing the increasing attention of researchers for its pivotal role in diverse pathological and physiological processes on grounds of robust oxidation and nitrification. Herein, we have successfully designed and synthesized a phenanthrenequinone benzyl borate-based chemosensor for fast and selective detection of ONOO-. The probe PTDP itself had an orange fluorescence, which was changed to strong blue fluorescence upon the addition of ONOO-, indicating the ratiometric response of the probe. This is so because of the cleavage of the benzyl boronate-protecting group of PTDP upon the addition of ONOO- with simultaneous releasing of pyridinyl-based chemosensor PPI. The PTDP showed outstanding performance in the various photophysical studies such as good selectivity, excellent sensitivity with a very low detection limit of 2.74 nM, and a very fast response time (<15 s). Furthermore, for practical applicability, it was successfully applied in the ratiometric detection of ONOO- in osteoblast precursor cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoblasts
/
Phenanthrenes
/
Peroxynitrous Acid
/
Fluorescent Dyes
Limits:
Animals
/
Humans
Language:
En
Journal:
Chem Res Toxicol
/
Chem. res. toxicol
/
Chemical research in toxicology
Journal subject:
TOXICOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
India
Country of publication:
Estados Unidos