An electrochemical aptasensor based on silver-thiolated graphene for highly sensitive detection of Pb2.
Anal Methods
; 16(18): 2905-2912, 2024 May 09.
Article
in En
| MEDLINE
| ID: mdl-38660709
ABSTRACT
The presence of lead ions (Pb2+) in the environment not only leads to environmental contamination but also poses a significant risk to public health through their migration into food and drinking water. Therefore, the development of rapid and effective techniques for detection of trace amounts of Pb2+ is crucial for safeguarding both the environment and biosafety. In this study, an aptamer-based electrochemical sensor was developed for specific detection of Pb2+ by modifying a polylysine (PLL) coated silver-thiolated graphene (Ag-SH-G) nanocomposite (PLL/Ag-SH-G) on the surface of a glassy carbon electrode, which was further modified with gold nanoparticles (AuNPs) for attachment of aptamers (Apt) that specifically recognized Pb2+. The Ag-SH-G particles were synthesized using a one-step in situ method, resulting in significantly enhanced electrochemical properties upon incorporating Ag nanoparticles into the PLL/Ag-SH-G composite. Coating of the covalently or no-covalently bonded Ag-SH-G particles with PLL provides an excellent supporting matrix, facilitating the assembly of AuNPs and a thiol-modified aptamer for Pb2+. Under optimized conditions, Apt/AuNPs/PLL/Ag-SH-G/GCE exhibited excellent sensing performance for Pb2+ with a wide linear response range (10-1000 nM), a low detection limit (0.047 nM) and extraordinary selectivity. The sensor was employed and satisfactory results were obtained in river water, soil and vegetable samples for the detection of Pb2+.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Silver
/
Aptamers, Nucleotide
/
Metal Nanoparticles
/
Electrochemical Techniques
/
Gold
/
Graphite
/
Lead
Language:
En
Journal:
Anal Methods
Year:
2024
Document type:
Article
Affiliation country:
China