Integrating recombinase polymerase amplification with CRISPR/Cas9-initiated nicking-rolling circle amplification in Staphylococcus aureus assay.
Chem Commun (Camb)
; 60(40): 5314-5317, 2024 May 14.
Article
in En
| MEDLINE
| ID: mdl-38666524
ABSTRACT
We integrate recombinase polymerase amplification (RPA) with CRISPR/Cas9-initiated nicking rolling circle amplification (CRISPR/Cas9-nRCA) for detecting Staphylococcus aureus. This approach utilizes a unique dimeric G-triplex structure, demonstrating firstly enhanced ThT fluorescence for target detection. The proof-of-concept study introduces a new avenue for integrating isothermal amplifications with CRISPR/Cas9 in the fields of pathogen detection and disease diagnosis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Staphylococcus aureus
/
Nucleic Acid Amplification Techniques
/
Recombinases
/
CRISPR-Cas Systems
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2024
Document type:
Article