Biodegradation of acetaminophen: Microcosm centric genomic-proteomic-metabolomics evidences.
Bioresour Technol
; 401: 130732, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38677386
ABSTRACT
Acetaminophen (APAP) is a frequently used, over-the-counter analgesic and antipyretic medication. Considering increase in global consumption, its ubiquity in environment with potential toxic impacts has become a cause of great concern. Hence, bioremediation of this emerging contaminant is of paramount significance. The present study incorporates a microcosm centric omics approach to gain in-depth insights into APAP degradation by Paracoccus sp. APAP_BH8. It can metabolize APAP (300 mg kg-1) within 16 days in soil microcosms. Genome analysis revealed potential genes capable of mediating degradation includes M20 aminoacylase family protein, guanidine deaminase, 4-hydroxybenzoate 3-monooxygenase, and 4-hydroxyphenylpyruvate dioxygenase. Whole proteome analysis showed differential expression of enzymes and bioinformatics provided evidence for stable binding of intermediates at the active site of considered enzymes. Metabolites identified were 4-aminophenol, hydroquinone, and 3-hydroxy-cis, cis-muconate. Therefore, Paracoccus sp. APAP_BH8 with versatile enzymatic and genetic attributes can be a promising candidate for formulating improved in situ APAP bioremediation strategies.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biodegradation, Environmental
/
Genomics
/
Proteomics
/
Acetaminophen
Language:
En
Journal:
Bioresour Technol
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2024
Document type:
Article
Affiliation country:
India
Country of publication:
Reino Unido