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Association Between Muscle Growth and Transcription of a Mutant MSTN Gene in Olive Flounder (Paralichthys olivaceus).
Kim, Ju-Won; Kim, Julan; Cho, Ja Young; Shin, Younhee; Son, Hyojung; Sathiyamoorthy, Subramaniyam; Kim, Bo-Seong; Kim, Young-Ok; Kang, Byeong-Chul; Kong, Hee Jeong.
Affiliation
  • Kim JW; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
  • Kim J; Genetics and Breeding Research Center, National Institute of Fisheries Science, Geoje, 53334, Republic of Korea.
  • Cho JY; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
  • Shin Y; Research and Development Center, Insilicogen Inc., Yongin-si, 16954, Republic of Korea.
  • Son H; Research and Development Center, Insilicogen Inc., Yongin-si, 16954, Republic of Korea.
  • Sathiyamoorthy S; Research and Development Center, Insilicogen Inc., Yongin-si, 16954, Republic of Korea.
  • Kim BS; Department of Aquatic Life Medicine, Kunsan National University, Gunsan, 54150, Republic of Korea.
  • Kim YO; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
  • Kang BC; D.iF Inc., Yongin-si, 16954, Republic of Korea. bckang@d-if.kr.
  • Kong HJ; Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea. heejkong@korea.kr.
Mar Biotechnol (NY) ; 26(3): 599-608, 2024 Jun.
Article in En | MEDLINE | ID: mdl-38683458
ABSTRACT
Myostatin (MSTN, also known as growth differentiation factor-8 (GDF-8)), a member of the transforming growth factor ß (TGF-ß) superfamily, functions as a negative regulator of skeletal muscle development and growth. However, it is also expressed in a wide range of tissues in fish and thus may have more diverse roles in this group than in mammals. In this study, we assessed the genome-wide transcriptional expression pattern associated with the CRISPR/Cas9-mutated MSTN gene in the olive flounder (Paralichthys olivaceus) in association with changes in cell proliferation and transportation processes. There were no differences in the hepatosomatic index, and the growth of male and female fish increased in the F1 progeny of the MSTN mutants. Furthermore, the histopathological analysis showed that myostatin editing resulted in a 41.24% increase in back muscle growth and 46.92% increase in belly muscle growth in male flounder compared with normal flounder, and a 16.01% increase in back muscle growth and 14.26% increase in belly muscle growth in female flounder compared with normal flounder. This study demonstrates that editing of the myostatin gene enhances muscle growth in olive flounder, with a notably more pronounced effect observed in males. Consequently, myostatin-edited male flounder could represent a valuable asset for the flounder aquaculture industry.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Flounder / Muscle, Skeletal / Myostatin Limits: Animals Language: En Journal: Mar Biotechnol (NY) Journal subject: BIOLOGIA / BIOTECNOLOGIA Year: 2024 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Flounder / Muscle, Skeletal / Myostatin Limits: Animals Language: En Journal: Mar Biotechnol (NY) Journal subject: BIOLOGIA / BIOTECNOLOGIA Year: 2024 Document type: Article
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