A paper-based point-of-care device for the detection of cysteine using gold nanoparticles from whole blood.
Anal Methods
; 16(19): 3007-3019, 2024 May 16.
Article
in En
| MEDLINE
| ID: mdl-38695537
ABSTRACT
We present a colorimetric probe based on polyvinylpyrrolidone-capped gold nanoparticles (PVP-AuNPs) that is sensitive and selective for cysteine (Cys). A microfluidic paper-based analytical device (µ-PAD) with embedded dried PVP-AuNPs at the polyethersulfone (PES) paper surface is used for Cys detection. When thiol molecules attach to PVP-AuNPs in the presence of Cys, they clump together, and this causes the solution's color to shift from red to blue within 5 minutes. The device is capable of detecting Cys levels between 1.0 µM and 50.0 µM with a limit of detection (LOD) of 0.2 µM under optimized conditions. The stability of the µ-PAD was tested for 100 days, demonstrating re-dispersibility to detect Cys levels in blood. Dried PVP-AuNP-µPADs were integrated with blood plasma separation modules for point-of-care (POC) Cys detection. Consequently, the device shows potential as a self-sustaining, quantification platform with a recovery percentage ranging from 98.44 to 111.9 in clinical samples.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Paper
/
Colorimetry
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Point-of-Care Systems
/
Cysteine
/
Metal Nanoparticles
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Limit of Detection
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Gold
Limits:
Humans
Language:
En
Journal:
Anal Methods
Year:
2024
Document type:
Article
Affiliation country:
India