Your browser doesn't support javascript.
loading
Comparative analysis of Streptococcus agalactiae serotypes Ia and II isolates from China and Pakistan in a murine model: A focus on pathogenesis and immune response.
Leghari, Ambreen; Sabir, Rabia; Laghari, Sheereen; Khand, Faiz Muhammad; Chandio, Muhammad Ali; Magsi, Abdul Samad; Bhutto, Khaliq Ur Rehman; Hassan, Mohammad Farooque; Lakho, Shakeel Ahmed; Lin, Huixing; Fan, Hongjie.
Affiliation
  • Leghari A; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sakrand, 67210, Sindh, Pakistan.
  • Sabir R; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
  • Laghari S; Department of Food Engineering, Quaid-e-Awam University of Engineering, Science & Technology Nawabshah 67450, Sindh, Pakistan.
  • Khand FM; Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sakrand, 67210, Sindh, Pakistan.
  • Chandio MA; Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sakrand, 67210, Sindh, Pakistan.
  • Magsi AS; Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sakrand, 67210, Sindh, Pakistan.
  • Bhutto KUR; Directorate of Veterinary Research and Diagnosis, CVDL, Sindh, 70050, Pakistan.
  • Hassan MF; Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sakrand, 67210, Sindh, Pakistan.
  • Lakho SA; Shaheed Benazir Bhutto University of Veterinary and Animal Sciences Sakrand, 67210, Sindh, Pakistan.
  • Lin H; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University
  • Fan H; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University
Microb Pathog ; 191: 106675, 2024 Jun.
Article in En | MEDLINE | ID: mdl-38705216
ABSTRACT
Bovine mastitis, caused by Streptococcus agalactiae (Group B Streptococcus; GBS), poses significant economic challenges to the global dairy industry. Mouse models serves as valuable tools for assessing GBS-induced infections as an alternative to large animals. This study aimed to investigate the LD50 dose, organ bacterial load, and quantification of peritoneal leukocyte populations for GBS serotypes Ia and II isolates from China and Pakistan. Additionally, we measured indicators such as lactoferrin, albumin, and myeloperoxidase (MPO) activity. Pro-inflammatory cytokines (TNF-α, IL-1ß, IL-6, and IL-2) and anti-inflammatory cytokines (IL-10 and TGF-ß) in serum and tissue samples were evaluated using ELISA and qPCR, respectively. BALB/c mice (4 mice per group) received individual intraperitoneal injections of 100 µl containing specific bacterial inoculum concentrations (ranging from 105 to 109 CFU per mouse) of Chinese and Pakistani GBS isolates (serotypes Ia and II). Control groups received 100 µL of sterile PBS. Results revealed that the LD50 bacterial dose causing 50 % mortality in mice was 107 CFU. The highest bacterial load in all experimental groups was quantified in the peritoneum, followed by blood, mammary gland, liver, spleen, lungs, and brain. The most significant bacterial dissemination was observed in mice inoculated with Pakistani serotype Ia at 24 h, with a subsequent notable decline in bacterial counts at day 3. Notably, infection with Pakistani serotype Ia showed a trend of increased total leukocyte counts, significantly higher than Pakistani serotype II, Chinese Serotype Ia, and Chinese serotype II. A substantial influx of neutrophils and lymphocytes was observed in response to all tested serotypes, with Pakistani serotype Ia inducing a significantly higher influx compared to other groups (Pakistani serotype II, Chinese serotype Ia, and Chinese serotype II). Furthermore, TNF-α, IL-1ß, IL-2, and IL-6 expressions were significantly increased in mice one day after infection with the Pakistani serotype Ia. Compared to mice infected with the Pakistani serotype II, Chinese Serotype Ia, and Chinese serotype II, those infected with the Pakistani serotype Ia isolate exhibited the highest production of IL-10 and TGF-ß, along with significantly increased concentrations of lactoferrin, albumin, and MPO. These findings suggest that the persistence and severity of infection caused by the Pakistani serotype Ia may be linked to its ability to spread to deeper tissues. This study enhances our understanding of the clinical characteristics of bovine mastitis caused by S. agalactiae in China and Pakistan.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Streptococcal Infections / Streptococcus agalactiae / Cytokines / Disease Models, Animal / Serogroup / Mice, Inbred BALB C Limits: Animals Country/Region as subject: Asia Language: En Journal: Microb Pathog Journal subject: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Year: 2024 Document type: Article Affiliation country: Pakistán Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Streptococcal Infections / Streptococcus agalactiae / Cytokines / Disease Models, Animal / Serogroup / Mice, Inbred BALB C Limits: Animals Country/Region as subject: Asia Language: En Journal: Microb Pathog Journal subject: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Year: 2024 Document type: Article Affiliation country: Pakistán Country of publication: Reino Unido