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Zooming into lipid droplet biology through the lens of electron microscopy.
Dudka, Wioleta; Salo, Veijo T; Mahamid, Julia.
Affiliation
  • Dudka W; Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
  • Salo VT; Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
  • Mahamid J; Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
FEBS Lett ; 598(10): 1127-1142, 2024 May.
Article in En | MEDLINE | ID: mdl-38726814
ABSTRACT
Electron microscopy (EM), in its various flavors, has significantly contributed to our understanding of lipid droplets (LD) as central organelles in cellular metabolism. For example, EM has illuminated that LDs, in contrast to all other cellular organelles, are uniquely enclosed by a single phospholipid monolayer, revealed the architecture of LD contact sites with different organelles, and provided near-atomic resolution maps of key enzymes that regulate neutral lipid biosynthesis and LD biogenesis. In this review, we first provide a brief history of pivotal findings in LD biology unveiled through the lens of an electron microscope. We describe the main EM techniques used in the context of LD research and discuss their current capabilities and limitations, thereby providing a foundation for utilizing suitable EM methodology to address LD-related questions with sufficient level of structural preservation, detail, and resolution. Finally, we highlight examples where EM has recently been and is expected to be instrumental in expanding the frontiers of LD biology.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Microscopy, Electron / Lipid Droplets Limits: Animals / Humans Language: En Journal: FEBS Lett Year: 2024 Document type: Article Affiliation country: Alemania

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Microscopy, Electron / Lipid Droplets Limits: Animals / Humans Language: En Journal: FEBS Lett Year: 2024 Document type: Article Affiliation country: Alemania