Structural characterization of squash polysaccharide and its effect on STZ-induced diabetes mellitus model in MIN6 cells.
Int J Biol Macromol
; 270(Pt 2): 132226, 2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38729469
ABSTRACT
A novel natural water-soluble acidic polysaccharide (PWESP-3) was isolated from squash with a molecular mass of 140.519â¯kDa, which was composed of arabinose (Ara, 35.30â¯mol%), galactose (Gal, 61.20â¯mol%), glucose (Glc, 1.80â¯mol%), and Mannuronic acid (ManA, 1.70â¯mol%) and contained Araf-(1â, â3)-Araf-(1â, â5)-Araf-(1â, Glcp-(1â, Galp-(1â, â3,5)-Araf-(1â, â2)-Glcp-(1â, â2)-Manp-(1â, â3)-Glcp-(1â, â4)-Galp-(1â, â3)-Galp-(1â, â6)-Galp-(1â, â3,4)-Galp-(1â, â4,6)-Galp-(1â residues in the backbone. Moreover, the structure of PWESP-3 was identified by NMR spectra. The branch chain was connected to the main chain by the O-3 and O-4 atom of Gal. In addition, the effect of PWESP-3 on STZ-induced type I diabetes mellitus model in MIN6 cells was investigated. The results showed that PWESP-3 can increase the viability and insulin secretion of MIN6 cells and reduce the oxidative stress caused by ROS and NO. Meanwhile, PWESP-3 can also reduce the content of ATP, Ca2+, mitochondrial membrane potential and Caspase-3 activity in MIN6 cells. Furthermore, treatment with PWESP-3 can prevent single or double stranded DNA breaking to form DNA fragments and improve DNA damage in MIN6 cells, thereby avoiding apoptosis. Therefore, the above data highlight that PWESP-3 can improve the function of insulin secretion in STZ-induced MIN6 cells in vitro and can be used as an alternative food supplement to diabetes drugs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides
/
Diabetes Mellitus, Experimental
Limits:
Animals
Language:
En
Journal:
Int J Biol Macromol
/
Int. j. biol. macromol
/
International journal of biological macromolecules
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Países Bajos