Genome-wide expression analysis of LACS gene family implies GhLACS25 functional responding to salt stress in cotton.
BMC Plant Biol
; 24(1): 392, 2024 May 13.
Article
in En
| MEDLINE
| ID: mdl-38735932
ABSTRACT
BACKGROUND:
Long-chain acyl-coenzyme A synthetase (LACS) is a type of acylating enzyme with AMP-binding, playing an important role in the growth, development, and stress response processes of plants.RESULTS:
The research team identified different numbers of LACS in four cotton species (Gossypium hirsutum, Gossypium barbadense, Gossypium raimondii, and Gossypium arboreum). By analyzing the structure and evolutionary characteristics of the LACS, the GhLACS were divided into six subgroups, and a chromosome distribution map of the family members was drawn, providing a basis for further research classification and positioning. Promoter cis-acting element analysis showed that most GhLACS contain plant hormones (GA, MeJA) or non-biological stress-related cis-elements. The expression patterns of GhLACS under salt stress treatment were analyzed, and the results showed that GhLACS may significantly participate in salt stress response through different mechanisms. The research team selected 12 GhLACSs responsive to salt stress for tissue expression analysis and found that these genes are expressed in different tissues.CONCLUSIONS:
There is a certain diversity of LACS among different cotton species. Analysis of promoter cis-acting elements suggests that GhLACS may be involved in regulating plant growth, development and stress response processes. GhLACS25 was selected for in-depth study, which confirmed its significant role in salt stress response through virus-induced gene silencing (VIGS) and induced expression in yeast cells.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Gossypium
/
Salt Stress
Language:
En
Journal:
BMC Plant Biol
/
BMC plant biol. (Online)
/
BMC plant biology (Online)
Journal subject:
BOTANICA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Reino Unido