The vacuolar fusion regulated by HOPS complex promotes hyphal initiation and penetration in Candida albicans.
Nat Commun
; 15(1): 4131, 2024 May 16.
Article
in En
| MEDLINE
| ID: mdl-38755250
ABSTRACT
The transition between yeast and hyphae is crucial for regulating the commensalism and pathogenicity in Candida albicans. The mechanisms that affect the invasion of hyphae in solid media, whose deficiency is more related to the pathogenicity of C. albicans, have not been elucidated. Here, we found that the disruption of VAM6 or VPS41 which are components of the homotypic vacuolar fusion and protein sorting (HOPS) complex, or the Rab GTPase YPT72, all responsible for vacuole fusion, led to defects in hyphal growth in both liquid and solid media, but more pronounced on solid agar. The phenotypes of vac8Δ/Δ and GTR1OE-vam6Δ/Δ mutants indicated that these deficiencies are mainly caused by the reduced mechanical forces that drive agar and organs penetration, and confirmed that large vacuoles are required for hyphal mechanical penetration. In summary, our study revealed that large vacuoles generated by vacuolar fusion support hyphal penetration and provided a perspective to refocus attention on the role of solid agar in evaluating C. albicans invasion.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vacuoles
/
Candida albicans
/
Fungal Proteins
/
Hyphae
/
Vesicular Transport Proteins
Limits:
Animals
Language:
En
Journal:
Nat Commun
/
Nature communications
Journal subject:
BIOLOGIA
/
CIENCIA
Year:
2024
Document type:
Article
Country of publication:
Reino Unido