All-in-one 2LabsToGo system for analysis of ergot alkaloids in whole rye.
Food Chem
; 453: 139593, 2024 Sep 30.
Article
in En
| MEDLINE
| ID: mdl-38761724
ABSTRACT
Ergot alkaloids, naturally occurring mycotoxins of Claviceps fungi, pose health risks. This necessitates accurate analysis methods to ensure food safety. This study explored the open-source miniaturized all-in-one 2LabsToGo system to analyze ergot alkaloids in whole rye samples. It is suited for sustainable atline analysis as it combines all planar chromatography tasks, allowing low-cost quality control in milling plants. The LOD and LOQ of ergocristine were determined to be 0.4 and 1.2 ng/zone, respectively. Detectability of ergot alkaloids was proven to be below the current maximum limit of 500 µg/kg for rye milling products. The repeatability (%RSD) was 4.1 % and the coefficient of determination of the analytical response (R2) was 0.9918 for ergocristine. The mean recovery rate of ergot alkaloids in spiked whole rye grain was close to 100 %. Results of screening whole rye for ergot alkaloids were successfully verified by comparison with those obtained by conventional status quo HPTLC instrumentation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Secale
/
Food Contamination
/
Ergot Alkaloids
Language:
En
Journal:
Food Chem
Year:
2024
Document type:
Article