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All-in-one 2LabsToGo system for analysis of ergot alkaloids in whole rye.
Jakob, Kevin; Schwack, Wolfgang; Morlock, Gertrud E.
Affiliation
  • Jakob K; Chair of Food Science, Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address: Kevin.Jakob@ernaehrung.uni-giessen.de.
  • Schwack W; Chair of Food Science, Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address: Wolfgang.Schwack@uni-giessen.de.
  • Morlock GE; Chair of Food Science, Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address: Gertrud.Morlock@uni-giessen.de.
Food Chem ; 453: 139593, 2024 Sep 30.
Article in En | MEDLINE | ID: mdl-38761724
ABSTRACT
Ergot alkaloids, naturally occurring mycotoxins of Claviceps fungi, pose health risks. This necessitates accurate analysis methods to ensure food safety. This study explored the open-source miniaturized all-in-one 2LabsToGo system to analyze ergot alkaloids in whole rye samples. It is suited for sustainable atline analysis as it combines all planar chromatography tasks, allowing low-cost quality control in milling plants. The LOD and LOQ of ergocristine were determined to be 0.4 and 1.2 ng/zone, respectively. Detectability of ergot alkaloids was proven to be below the current maximum limit of 500 µg/kg for rye milling products. The repeatability (%RSD) was 4.1 % and the coefficient of determination of the analytical response (R2) was 0.9918 for ergocristine. The mean recovery rate of ergot alkaloids in spiked whole rye grain was close to 100 %. Results of screening whole rye for ergot alkaloids were successfully verified by comparison with those obtained by conventional status quo HPTLC instrumentation.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Secale / Food Contamination / Ergot Alkaloids Language: En Journal: Food Chem Year: 2024 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Secale / Food Contamination / Ergot Alkaloids Language: En Journal: Food Chem Year: 2024 Document type: Article