Effect of cevimeline and different concentration of gum arabic on parotid salivary gland function in methotrexate-induced xerostomia: a comparative study.
BMC Oral Health
; 24(1): 624, 2024 May 28.
Article
in En
| MEDLINE
| ID: mdl-38807094
ABSTRACT
OBJECTIVE:
This study assessed the effect of cevimeline and different concentrations of gum arabic on the parotid gland of rats being given xerostomia-inducing methotrexate.METHODS:
One hundred twenty-five rats were divided into five equal groups of twenty-five each. The rats in Group I received basic diets, while those in Groups II, III, IV, and V received 20 mg/kg MTX as a single intraperitoneal dose on day one. Group III received 10 mg/kg CVM dissolved in saline orally and daily, and the other two groups received a 10% W/V aqueous suspension of GA. Therefore, Group IV received 2 ml/kg suspension orally and daily, while Group V received 3 ml/kg suspension orally and daily. After 9 days, the parotid glands were dissected carefully and prepared for hematoxylin and eosin (H&E) staining as a routine histological stain and caspase-3 and Ki67 immunohistochemical staining. Quantitative data from α-Caspase-3 staining and Ki67 staining were statistically analysed using one-way ANOVA followed by Tukey's multiple comparisons post hoc test.RESULTS:
Regarding caspase-3 and Ki67 immunohistochemical staining, one-way ANOVA revealed a significant difference among the five groups. For Caspase-3, the highest mean value was for group II (54.21 ± 6.90), and the lowest mean value was for group I (15.75 ± 3.67). The other three groups had mean values of 31.09 ± 5.90, 30.76 ± 5.82, and 20.65 ± 3.47 for groups III, IV, and V, respectively. For Ki67, the highest mean value was for group I (61.70 ± 6.58), and the lowest value was for group II (18.14a ± 5.16). The other three groups had mean values of 34.4 ± 9.27, 48.03 ± 8.40, and 50.63 ± 8.27 for groups III, IV, and V, respectively.CONCLUSION:
GA, rather than the normally used drug CVM, had a desirable effect on the salivary glands of patients with xerostomia.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Parotid Gland
/
Thiophenes
/
Xerostomia
/
Methotrexate
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Ki-67 Antigen
/
Gum Arabic
Limits:
Animals
Language:
En
Journal:
BMC Oral Health
Journal subject:
ODONTOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Egipto
Country of publication:
Reino Unido