Glass bead system to study mycotoxin production of Aspergillus spp. on corn and rice starches.
Appl Microbiol Biotechnol
; 108(1): 348, 2024 May 29.
Article
in En
| MEDLINE
| ID: mdl-38809353
ABSTRACT
Mycotoxin production by aflatoxin B1 (AFB1) -producing Aspergillus flavus Zt41 and sterigmatocystin (ST) -hyperproducer Aspergillus creber 2663 mold strains on corn and rice starch, both of high purity and nearly identical amylose-amylopectin composition, as the only source of carbon, was studied. Scanning electron microscopy revealed average starch particle sizes of 4.54 ± 0.635 µm and 10.9 ± 2.78 µm, corresponding to surface area to volume ratios of 127 1/µm for rice starch and 0.49 1/µm for corn starch. Thus, a 2.5-fold difference in particle size correlated to a larger, 259-fold difference in surface area. To allow starch, a water-absorbing powder, to be used as a sole food source for Aspergillus strains, a special glass bead system was applied. AFB1 production of A. flavus Zt41 was determined to be 437.6 ± 128.4 ng/g and 90.0 ± 44.8 ng/g on rice and corn starch, respectively, while corresponding ST production levels by A. creber 2663 were 72.8 ± 10.0 µg/g and 26.8 ± 11.6 µg/g, indicating 3-fivefold higher mycotoxin levels on rice starch than on corn starch as sole carbon and energy sources. KEY POINTS ⢠A glass bead system ensuring the flow of air when studying powders was developed. ⢠AFB1 and ST production of A. flavus and A. creber on rice and corn starches were studied. ⢠3-fivefold higher mycotoxin levels on rice starch than on corn starch were detected.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oryza
/
Starch
/
Zea mays
Language:
En
Journal:
Appl Microbiol Biotechnol
Year:
2024
Document type:
Article
Affiliation country:
Hungria
Country of publication:
Alemania