Evolution of diapause in the African turquoise killifish by remodeling the ancient gene regulatory landscape.
Cell
; 187(13): 3338-3356.e30, 2024 Jun 20.
Article
in En
| MEDLINE
| ID: mdl-38810644
ABSTRACT
Suspended animation states allow organisms to survive extreme environments. The African turquoise killifish has evolved diapause as a form of suspended development to survive a complete drought. However, the mechanisms underlying the evolution of extreme survival states are unknown. To understand diapause evolution, we performed integrative multi-omics (gene expression, chromatin accessibility, and lipidomics) in the embryos of multiple killifish species. We find that diapause evolved by a recent remodeling of regulatory elements at very ancient gene duplicates (paralogs) present in all vertebrates. CRISPR-Cas9-based perturbations identify the transcription factors REST/NRSF and FOXOs as critical for the diapause gene expression program, including genes involved in lipid metabolism. Indeed, diapause shows a distinct lipid profile, with an increase in triglycerides with very-long-chain fatty acids. Our work suggests a mechanism for the evolution of complex adaptations and offers strategies to promote long-term survival by activating suspended animation programs in other species.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Diapause
Limits:
Animals
Language:
En
Journal:
Cell
Year:
2024
Document type:
Article
Country of publication:
Estados Unidos