Haplotype-resolved gapless genome and chromosome segment substitution lines facilitate gene identification in wild rice.
Nat Commun
; 15(1): 4573, 2024 May 29.
Article
in En
| MEDLINE
| ID: mdl-38811581
ABSTRACT
The abundant genetic variation harbored by wild rice (Oryza rufipogon) has provided a reservoir of useful genes for rice breeding. However, the genome of wild rice has not yet been comprehensively assessed. Here, we report the haplotype-resolved gapless genome assembly and annotation of wild rice Y476. In addition, we develop two sets of chromosome segment substitution lines (CSSLs) using Y476 as the donor parent and cultivated rice as the recurrent parents. By analyzing the gapless reference genome and CSSL population, we identify 254 QTLs associated with agronomic traits, biotic and abiotic stresses. We clone a receptor-like kinase gene associated with rice blast resistance and confirm its wild rice allele improves rice blast resistance. Collectively, our study provides a haplotype-resolved gapless reference genome and demonstrates a highly efficient platform for gene identification from wild rice.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oryza
/
Haplotypes
/
Genome, Plant
/
Chromosomes, Plant
/
Quantitative Trait Loci
Language:
En
Journal:
Nat Commun
/
Nature communications
Journal subject:
BIOLOGIA
/
CIENCIA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Reino Unido