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The activation of AMPK/PGC-1α/GLUT4 signaling pathway through early exercise improves mitochondrial function and mitigates ischemic brain damage.
Wu, Xinyue; Li, Chen; Ke, Changkai; Huang, Chuan; Pan, Bingchen; Wan, Chunxiao.
Affiliation
  • Wu X; Department of Rehabilitation Medicine, Tianjin Medical University General Hospital.
  • Li C; Department of Rehabilitation Medicine, Tianjin Medical University General Hospital.
  • Ke C; Department of Rehabilitation Medicine, Tianjin Medical University General Hospital.
  • Huang C; Department of Rehabilitation Medicine, Tianjin Medical University General Hospital.
  • Pan B; Institute of Medical technology, Tianjin Medical University, Tianjin, China.
  • Wan C; Department of Rehabilitation Medicine, Tianjin Medical University General Hospital.
Neuroreport ; 35(10): 648-656, 2024 07 01.
Article in En | MEDLINE | ID: mdl-38813901
ABSTRACT
Mitochondria play a crucial role in maintaining cellular energy supply and serve as a source of energy for repairing nerve damage following a stroke. Given that exercise has the potential to enhance energy metabolism, investigating the impact of exercise on mitochondrial function provides a plausible mechanism for stroke treatment. In our study, we established the middle cerebral artery occlusion (MCAO) model in Sprague-Dawley rats and implemented early exercise intervention. Neurological severity scores, beam-walking test score, and weight were used to evaluate neurological function. The volume of cerebral infarction was measured by MRI. Nerve cell apoptosis was detected by TUNEL staining. Mitochondrial morphology and structure were detected by mitochondrial electron microscopy. Mitochondrial function was assessed using membrane potential and ATP measurements. Western blotting was used to detect the protein expression of AMPK/PGC-1α/GLUT4. Through the above experiments, we found that early exercise improved neurological function in rats after MCAO, reduced cerebral infarction volume and neuronal apoptosis, promoted the recovery of mitochondrial morphology and function. We further examined the protein expression of AMPK/PGC-1α/GLUT4 signaling pathway and confirmed that early exercise was able to increase its expression. Therefore, we suggest that early exercise initiated the AMPK/PGC-1α/GLUT4 signaling pathway, restoring mitochondrial function and augmenting energy supply. This, in turn, effectively improved both nerve and body function in rats following ischemic stroke.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Physical Conditioning, Animal / Signal Transduction / Rats, Sprague-Dawley / AMP-Activated Protein Kinases / Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Mitochondria Limits: Animals Language: En Journal: Neuroreport Journal subject: NEUROLOGIA Year: 2024 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Physical Conditioning, Animal / Signal Transduction / Rats, Sprague-Dawley / AMP-Activated Protein Kinases / Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Mitochondria Limits: Animals Language: En Journal: Neuroreport Journal subject: NEUROLOGIA Year: 2024 Document type: Article