The organisation of the digestive, excretory and reproductive systems in cysts of the freshwater tardigrade Thulinius ruffoi (Parachela, Isohypsibioidea: Doryphoribiidae).

ABSTRACT

Tardigrades are invertebrates known to science for over 250 years. Although the ability of some species of tardigrades to form cysts has been reported, little is known about the encystment and internal organisation of the cysts. During cyst formation, contraction of the body affects the internal organs' morphology. The organs are compressed and have a compact appearance. The organisation of the digestive system, associated structures, and the reproductive system are analysed in cysts on indefinite and well-defined encystment periods - up to eleven months. The digestive system of encysted animals was organised into three main parts - a foregut, a midgut, and a hindgut. The presence of digestive system-associated structures, such as buccal glands or muscles, was noted and described. The excretory organs, called Malpighian tubules, open into the zone between the midgut and the hindgut. Furthermore, the oviduct opens into the hindgut. The first analysis of the reproductive system of cysts at the ultrastructural level is presented here, revealing interesting and undescribed aspects related to the physiology. Besides the anatomical and histological examination, the morphology and changes that occur during cyst formation are described.