Vertebrate centromere architecture: from chromatin threads to functional structures.
Chromosoma
; 133(3): 169-181, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38856923
ABSTRACT
Centromeres are chromatin structures specialized in sister chromatid cohesion, kinetochore assembly, and microtubule attachment during chromosome segregation. The regional centromere of vertebrates consists of long regions of highly repetitive sequences occupied by the Histone H3 variant CENP-A, and which are flanked by pericentromeres. The three-dimensional organization of centromeric chromatin is paramount for its functionality and its ability to withstand spindle forces. Alongside CENP-A, key contributors to the folding of this structure include components of the Constitutive Centromere-Associated Network (CCAN), the protein CENP-B, and condensin and cohesin complexes. Despite its importance, the intricate architecture of the regional centromere of vertebrates remains largely unknown. Recent advancements in long-read sequencing, super-resolution and cryo-electron microscopy, and chromosome conformation capture techniques have significantly improved our understanding of this structure at various levels, from the linear arrangement of centromeric sequences and their epigenetic landscape to their higher-order compaction. In this review, we discuss the latest insights on centromere organization and place them in the context of recent findings describing a bipartite higher-order organization of the centromere.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vertebrates
/
Chromatin
/
Chromosomal Proteins, Non-Histone
/
Centromere
Limits:
Animals
/
Humans
Language:
En
Journal:
Chromosoma
Year:
2024
Document type:
Article
Affiliation country:
Países Bajos