Genome organization regulates nuclear pore complex formation and promotes differentiation during Drosophila oogenesis.
Genes Dev
; 38(9-10): 436-454, 2024 Jun 25.
Article
in En
| MEDLINE
| ID: mdl-38866556
ABSTRACT
Genome organization can regulate gene expression and promote cell fate transitions. The differentiation of germline stem cells (GSCs) to oocytes in Drosophila involves changes in genome organization mediated by heterochromatin and the nuclear pore complex (NPC). Heterochromatin represses germ cell genes during differentiation, and NPCs anchor these silenced genes to the nuclear periphery, maintaining silencing to allow for oocyte development. Surprisingly, we found that genome organization also contributes to NPC formation, mediated by the transcription factor Stonewall (Stwl). As GSCs differentiate, Stwl accumulates at boundaries between silenced and active gene compartments. Stwl at these boundaries plays a pivotal role in transitioning germ cell genes into a silenced state and activating a group of oocyte genes and nucleoporins (Nups). The upregulation of these Nups during differentiation is crucial for NPC formation and further genome organization. Thus, cross-talk between genome architecture and NPCs is essential for successful cell fate transitions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oogenesis
/
Cell Differentiation
/
Nuclear Pore
/
Drosophila Proteins
/
Genome, Insect
Limits:
Animals
Language:
En
Journal:
Genes Dev
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
Estados Unidos