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Polytyrosine-Coated Paper Electrode for Sensitive and Selective Sensing of NADH.
Jain, Supriya; Sonia, Joseph; Prashanth, Sharmila; Sanjeeva, Sandesh G; Prasad, K Sudhakara; Johnson, Renjith P.
Affiliation
  • Jain S; Polymer Nanobiomaterial Research Laboratory, Smart Materials and Devices, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575 018, India.
  • Sonia J; Nanomaterial Research Laboratory (NMRL), Smart Materials and Devices, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore 575 018, India.
  • Prashanth S; Nanomaterial Research Laboratory (NMRL), Smart Materials and Devices, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore 575 018, India.
  • Sanjeeva SG; Polymer Nanobiomaterial Research Laboratory, Smart Materials and Devices, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575 018, India.
  • Prasad KS; Nanomaterial Research Laboratory (NMRL), Smart Materials and Devices, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore 575 018, India.
  • Johnson RP; Centre for Nutrition Studies, Yenepoya (Deemed to be University), Deralakatte, Mangalore 575 018, India.
Langmuir ; 40(25): 13144-13154, 2024 Jun 25.
Article in En | MEDLINE | ID: mdl-38869442
ABSTRACT
Reduced nicotinamide adenine dinucleotide (NADH)-detecting electrochemical sensors are attractive in monitoring and diagnosing various physiological disorders of NADH abnormalities. The NADH detection methods using conventional electrodes are challenging due to slow electron transfer and fouling effect. Interestingly, paper-based flexible and disposable electrodes (PE) are superior for sensing biomolecules through simple detection procedures with excellent sensitivity and selectivity. Herein, to construct a conducting polypeptide-modified paper electrode, initially, polytyrosine (PTyr) is synthesized from l-tyrosine N-carboxy anhydride through ring-opening polymerization, and PTyr is drop-coated on the PE. The PTyr-modified paper electrode (PMPE) demonstrated excellent electrochemical properties and facilitated the electrooxidation of NADH at a lower potential of 576 mV. The PMPE displayed a linear detection between 25 and 145 µM of NADH concentration, with a lower detection limit of 0.340 µM. Under ideal circumstances, the sensor developed displayed an excellent NADH detection capability without interference with the most common electroactive species, ascorbic acid. The PMPE facilitates good electrocatalytic activity toward NADH, which can also be employed as a substrate material for biofuel cells.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Paper / Electrodes / NAD Language: En Journal: Langmuir Journal subject: QUIMICA Year: 2024 Document type: Article Affiliation country: India

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Paper / Electrodes / NAD Language: En Journal: Langmuir Journal subject: QUIMICA Year: 2024 Document type: Article Affiliation country: India