Neurite outgrowth promotion in PC12 cells by 24(R)-ethyllophenol from Morus alba.
Pharmazie
; 79(3): 67-71, 2024 May 15.
Article
in En
| MEDLINE
| ID: mdl-38872272
ABSTRACT
We examined the mechanism by which 24(R)-ethyllophenol (MAB28) isolated from the branches of Morus alba caused neurite outgrowth in rat pheochromocytoma cells (PC12). MAB28 significantly promoted neurite outgrowth to a similar degree as the positive control, nerve growth factor (NGF). After incubation with MAB28 in PC12 cells, phosphorylation of extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, and cyclic AMP response element-binding protein was detected, but the time course of phosphorylation was different from that induced by NGF. The expression of chloride intracellular channel protein 3 (CLIC3) was significantly decreased by MAB28. 5-Nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB), an outward rectifying chloride channel inhibitor, significantly promoted neurite outgrowth in PC12 cells. These data suggested that MAB28 could induce neurite outgrowth by downregulating CLIC3 expression.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Neurites
/
Morus
Limits:
Animals
Language:
En
Journal:
Pharmazie
Journal subject:
FARMACIA
Year:
2024
Document type:
Article
Affiliation country:
Japón
Country of publication:
Alemania