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Gelatin nanoparticles loaded with 3-alkylpyridinium salt APS7, an analog of marine toxin, are a promising support in human lung cancer therapy.
Kononenko, Veno; Joukhan, Ahmad; Bele, Tadeja; Krizaj, Igor; Kralj, Slavko; Turk, Tom; Drobne, Damjana.
Affiliation
  • Kononenko V; Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, Ljubljana SI-1000, Slovenia. Electronic address: veno.kononenko@bf.uni-lj.si.
  • Joukhan A; Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, Ljubljana SI-1000, Slovenia; Faculty of Pharmacy, University of Ljubljana, Askerceva cesta 7, Ljubljana SI-1000, Slovenia.
  • Bele T; Department of Molecular and Biomedical Sciences, Jozef Stefan Institute, Jamova 39, Ljubljana SI-1000, Slovenia; Faculty of medicine, University of Ljubljana, Vrazov trg 2, Ljubljana SI-1000, Slovenia.
  • Krizaj I; Department of Molecular and Biomedical Sciences, Jozef Stefan Institute, Jamova 39, Ljubljana SI-1000, Slovenia.
  • Kralj S; Department for Materials Synthesis, Jozef Stefan Institute, Jamova 39, Ljubljana SI-1000, Slovenia.
  • Turk T; Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, Ljubljana SI-1000, Slovenia.
  • Drobne D; Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, Ljubljana SI-1000, Slovenia. Electronic address: damjana.drobne@bf.uni-lj.si.
Biomed Pharmacother ; 177: 117007, 2024 Aug.
Article in En | MEDLINE | ID: mdl-38906020
ABSTRACT
This study demonstrates the potential of gelatin nanoparticles as a nanodelivery system for antagonists of nicotinic acetylcholine receptors (nAChRs) to improve chemotherapy efficacy and reduce off-target effects. Too often, chemotherapy for lung cancer does not lead to satisfactory results. Therefore, new approaches directed at multiple pharmacological targets in cancer therapy are being developed. Following the activation of nAChRs (e.g. by nicotine), cancer cells begin to proliferate and become more resistant to chemotherapy-induced apoptosis. This work shows that the 3-alkylpyridinium salt, APS7, a synthetic analog of a toxin from the marine sponge Haliclona (Rhizoneira) sarai, acts as an nAChR antagonist that inhibits the pro-proliferative and anti-apoptotic effects of nicotine on A549 human lung adenocarcinoma cells. In this study, gelatin-based nanoparticles filled with APS7 (APS7-GNPs) were prepared and their effects on A549 cells were compared with that of free APS7. Both APS7 and APS7-GNPs inhibited Ca2+ influx and increased the efficacy of cisplatin chemotherapy in nicotine-stimulated A549 cells. However, significant benefits from APS7-GNPs were observed - a stronger reduction in the proliferation of A549 lung cancer cells and a much higher selectivity in cytotoxicity towards cancer cells compared with non-tumorigenic lung epithelial BEAS-2B cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Proliferation / Nanoparticles / Gelatin / Lung Neoplasms Limits: Humans Language: En Journal: Biomed Pharmacother Year: 2024 Document type: Article Country of publication: Francia

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Proliferation / Nanoparticles / Gelatin / Lung Neoplasms Limits: Humans Language: En Journal: Biomed Pharmacother Year: 2024 Document type: Article Country of publication: Francia