Capture of armA by a novel ISCR element, ISCR28.
Int J Antimicrob Agents
; 64(3): 107250, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-38908532
ABSTRACT
ISCR28 is a fully functional and active member of the IS91-like family of insertion sequences. ISCR28 is 1,708-bp long and contains a 1,293-bp long putative open reading frame that codes a transposase. Sixty ISCR28-containing sequences from GenBank generated 27 non-repeat genetic contexts, all of which represented naturally occurring biological events that had occurred in a wide range of gram-negative organisms. Insertion of ISCR28 into target DNA preferred the presence of a 5'-GXXT-3' sequence at its terIS (replication terminator) end. Loss of the first 4 bp of its oriIS (origin of replication) likely caused ISCR28 to be trapped in ISApl1-based transposons or similar structures. Loss of terIS and fusion with a mobile element upstream likely promoted co-transfer of ISCR28 and the downstream resistance genes. ArmA and its downstream intact ISCR28 can be excised from recombinant pKD46 plasmids forming circular intermediates, further elucidating its activity as a transposase.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Transposable Elements
/
Transposases
Language:
En
Journal:
Int J Antimicrob Agents
/
Int. j. antimicrob. agents
/
International journal of antimicrobial agents
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Países Bajos