Establishment and evaluation of a qPCR method for the detection of pfmdr1 mutations in Plasmodium falciparum, the causal agent of fatal malaria.
Diagn Microbiol Infect Dis
; 110(1): 116400, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-38909426
ABSTRACT
Drug resistance surveillance is a major integral part of malaria control programs. Molecular methods play a pivotal role in drug resistance detection and related molecular research. This study aimed to develop a rapid and accurate detection method for drug resistance of Plasmodium falciparum (P. falciparum). A quantitative real-time PCR (qPCR) assay has been developed that identifies the mutation at locus A256T in the P.falciparum multi-drug resistance(pfmdr1) gene producing amino acid change at position 86. The results of 198 samples detected by qPCR were consistent with nested PCR and sequencing, giving an accuracy of 94.3%. The sensitivity, specificity, positive and negative predictive value of qPCR were 85.7%, 97.6%, 90.0% and 96.4%, respectively. The results of qPCR are basically consistent with the nested PCR, which is expected to replace the nested PCR as a new molecular biological method for drug resistance detection, providing reliable technical support for global malaria prevention and control.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmodium falciparum
/
Sensitivity and Specificity
/
Malaria, Falciparum
/
Multidrug Resistance-Associated Proteins
/
Real-Time Polymerase Chain Reaction
Limits:
Humans
Language:
En
Journal:
Diagn Microbiol Infect Dis
/
Diagn. microbiol. infect. dis
/
Diagnostic microbiology and infectious disease
Year:
2024
Document type:
Article
Country of publication:
Estados Unidos