First Indonesian report of WGS-based MTBC L3 discovery.
BMC Res Notes
; 17(1): 176, 2024 Jun 25.
Article
in En
| MEDLINE
| ID: mdl-38915046
ABSTRACT
OBJECTIVE:
Recent spoligotyping results in the island nation of Indonesia had revealed the existence of Mycobacterium tuberculosis complex lineage 3 (MTBC L3) or Central Asian (CAS) strains. In this work, whole-genome sequencing (WGS) - based methods were used to search for the presence of MTBC L3.RESULTS:
Two unrelated Indonesian L3 strains discovered by WGS-based SNP phylogenomics are presented here for the first time. Assemblies of their genomes yielded 96.95% (MTBC strain Mtb_S6970) and 98.35% (Mtb_S19106) of the known reference strain H37Rv. Their respective constructed genome coverages are 45.38 ± 12.95x and 63.13 ± 21.10x. The two L3 genomes have 4062 and 4121 genes, respectively, which are well within the number of genes predicted in MTBC strains. Instead of having three rRNA genes usually, Mtb_S6970 possesses four. These L3 isolates exhibit cross-class antibiotic susceptibility. FadD26, fadE24, fbpA, lprO, and panC, which are thought to be important in the pathophysiology of MTBC, were discovered to have 3-7 times more loci in L3 than L2 or L4. The penetration of L3 in the nation, despite its antibiotic sensitivity, is a concerning indicator of borderless global spread that may eventually be overcome by the phenotypes of acquired drug resistance.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genome, Bacterial
/
Whole Genome Sequencing
/
Mycobacterium tuberculosis
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
BMC Res Notes
Year:
2024
Document type:
Article
Affiliation country:
Indonesia