In vitro fermentation reveals an interplay relationship between oat ß-glucan and human gut Bacteroides and their potential role in regulating gut cytokines.
Food Funct
; 15(15): 7794-7811, 2024 Jul 29.
Article
in En
| MEDLINE
| ID: mdl-38920001
ABSTRACT
Dietary oat ß-glucan regulates the gut microbial composition and structure; however, the interplay relationship between oat ß-glucan and the gut microbiota is unclear. In this study, we aim to investigate the interaction between oat ß-glucan and human gut Bacteroides, a versatile carbohydrate utilizer, and explore the effect of their interaction on gut immunity homeostasis. The results of in vitro fermentation showed that oat ß-glucan significantly increased the abundance of gut Bacteroides at the genus level. Then, Bacteroides strains were isolated from human gut microbiota and 9 strains of Bacteroides could grow on oat ß-glucan and degrade oat ß-glucan to reducing sugars. Notably, strains Bacteroides xylanisolvens Bac02 and Bacteroides koreensis Bac08 possessed the strongest degradation capacity towards oat ß-glucan. Genome analysis and functional annotations suggested that B. xylanisolvens Bac02 and B. koreensis Bac08 contained abundant genes encoding glycoside hydrolases family 3 (GH3) and GH16, which might be responsible for ß-glucan degradation. Moreover, cell experiments revealed that the metabolites from oat ß-glucan fermentation by these 9 strains of Bacteroides could regulate the polarization of macrophages and maintain gut immunity homeostasis. Our study provides a novel insight into research on the interplay between dietary compounds and the gut microbiota.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacteroides
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Cytokines
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Avena
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Beta-Glucans
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Fermentation
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Gastrointestinal Microbiome
Limits:
Animals
/
Humans
Language:
En
Journal:
Food Funct
Year:
2024
Document type:
Article
Affiliation country:
China