Svornostia abyssi gen. nov., sp. nov. isolated from the world's deepest silver-uranium mine currently devoted to the extraction of radon-saturated water.
Int J Syst Evol Microbiol
; 74(6)2024 Jun.
Article
in En
| MEDLINE
| ID: mdl-38922323
ABSTRACT
A Gram-stain-positive, rod-shaped, aerobic, motile bacterium, J379T, was isolated from radioactive water spring C1, located in a former silver-uranium mine in the Czech Republic. This slow-growing strain exhibited optimal growth at 24-28â°C on solid media with <1â% salt concentration and alkaline pH 8-10. The only respiratory quinone found in strain J379T was MK-7(H4). C18ââ1 ω9c (60.9â%), C18ââ0 (9.4â%), C16ââ0 and alcohol-C18ââ0 (both 6.2â%) were found to be the major fatty acids. The peptidoglycan contained directly cross-linked meso-diaminopimelic acid. Phylogenetic reconstruction based on the 16S rRNA gene sequences and the core-genome analysis revealed that strain J379T forms a separate phylogenetic lineage within the recently amended order Solirubrobacterales. A comparison of the 16S rRNA gene sequences between strain J379T and other members of the order Solirubrobacterales showed <96â% similarity. This analysis revealed that the closest type strains were Parviterribacter kavangonensis D16/0 /H6T (95.2â%), Capillimicrobium parvum 0166_1T (94.9â%) and Conexibacter arvalis KV-962T (94.5â%). Whole-genome analysis showed that the closest type strain was Baekduia soli BR7-21T with an average nucleotide identity of 78â%, average amino acid identity of 63.2â% and percentage of conserved proteins of 48.2â%. The G+C content of the J379T genomic DNA was 71.7âmol%. Based on the phylogenetic and phylogenomic data, as well as its physiological characteristics, strain J379T is proposed to represent a type strain (DSM 113746T=CCM 9300T) of Svornostia abyssi gen. nov. sp. nov. within the family Baekduiaceae.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Base Composition
/
DNA, Bacterial
/
RNA, Ribosomal, 16S
/
Bacterial Typing Techniques
/
Sequence Analysis, DNA
/
Fatty Acids
/
Mining
Country/Region as subject:
Europa
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
República Checa
Country of publication:
Reino Unido