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Cytotoxic impact of nicotine products on periodontal ligament cells.
Wiesmann-Imilowski, Nadine; Becker, Philipp; Gielisch, Matthias W; Ziebolz, Dirk; Vermehren, Franziska; Bitschnau, Marian; Langguth, Nils; Brieger, Jürgen; Deschner, James; Kämmerer, Peer W.
Affiliation
  • Wiesmann-Imilowski N; Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
  • Becker P; Department of Otorhinolaryngology, University Medical Center Mainz, Langenbeckstrasse 1, 55131, Mainz, Germany.
  • Gielisch MW; Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
  • Ziebolz D; Department of Oral and Maxillofacial Surgery, Federal Armed Forces Hospital, Ruebenacherstr. 170, 56072, Koblenz, Germany.
  • Vermehren F; Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
  • Bitschnau M; Department of Cariology, Endodontology and Periodontology, University of Leipzig, 04103, Leipzig, Germany.
  • Langguth N; Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
  • Brieger J; Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
  • Deschner J; Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
  • Kämmerer PW; Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
Clin Oral Investig ; 28(7): 399, 2024 Jun 26.
Article in En | MEDLINE | ID: mdl-38922383
ABSTRACT

OBJECTIVES:

The primary objective of this in vitro experiment was an assessment of proliferative capacity, metabolic activity, and potential cellular detriment of human periodontal ligament cells (hPDL) exposed to cigarette smoke (CS), electronic cigarette vapor (eCV), and heated tobacco product aerosol (HTP), or air (control). MATERIALS AND

METHODS:

Using a CAD/CAM-designed exposition chamber, hPDL were exposed to CS, eCV, HTP, or air (control) based on the Health Canada Intense Smoking Regime. Cell proliferation, metabolic activity, and cellular detriment were assessed at various time points.

RESULTS:

Compared to the control, hPDL exposed to CS exhibited significantly decreased cell numbers at all time points. HTP exposure led to reduced cell numbers 48 h and 72 h post-exposure, while eCV-exposed cells showed no significant decrease. The metabolic activity of eCV-treated hPDL was slightly reduced at 7 h but recovered at 24 h and 48 h. In contrast, CS-treated cells exhibited significantly decreased metabolic activity at 24 h and 48 h, and HTP-exposed cells showed a significant decrease after 48 h. Flow cytometry indicated both apoptotic and necrotic cell death following CS exposure, with necrotic cell death being more pronounced.

CONCLUSIONS:

eCV and HTP demonstrated comparatively reduced detrimental effects on hPDL compared to CS. CLINICAL RELEVANCE The findings suggest that conventional cigarette smoke poses a substantial risk to periodontal health by significantly impairing cell proliferation and metabolic activity. However, alternatives such as eCV and HTP may offer a comparatively reduced risk.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Periodontal Ligament / Cell Proliferation / Tobacco Products / Electronic Nicotine Delivery Systems Limits: Humans Language: En Journal: Clin Oral Investig Journal subject: ODONTOLOGIA Year: 2024 Document type: Article Affiliation country: Alemania Country of publication: Alemania

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Periodontal Ligament / Cell Proliferation / Tobacco Products / Electronic Nicotine Delivery Systems Limits: Humans Language: En Journal: Clin Oral Investig Journal subject: ODONTOLOGIA Year: 2024 Document type: Article Affiliation country: Alemania Country of publication: Alemania