Discovery and characterisation of new phage targeting uropathogenic Escherichia coli.
Virology
; 597: 110148, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-38941748
ABSTRACT
Antimicrobial resistance is an escalating threat with few new therapeutic options in the pipeline. Urinary tract infections (UTIs) are one of the most prevalent bacterial infections globally and are prone to becoming recurrent and antibiotic resistant. We discovered and characterized six novel Autographiviridae and Guernseyvirinae bacterial viruses (phage) against uropathogenic Escherichia coli (UPEC), a leading cause of UTIs. The phage genomes were between 39,471 bp - 45,233 bp, with 45.0%-51.0% GC%, and 57-84 predicted coding sequences per genome. We show that tail fiber domain structure, predicted host capsule type, and host antiphage repertoire correlate with phage host range. In vitro characterisation of phage cocktails showed synergistic improvement against a mixed UPEC strain population and when sequentially dosed. Together, these phage are a new set extending available treatments for UTI from UPEC, and phage vM_EcoM_SHAK9454 represents a promising candidate for further improvement through engineering.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacteriophages
/
Urinary Tract Infections
/
Genome, Viral
/
Escherichia coli Infections
/
Uropathogenic Escherichia coli
/
Host Specificity
/
Phage Therapy
Limits:
Humans
Language:
En
Journal:
Virology
Year:
2024
Document type:
Article
Affiliation country:
Australia
Country of publication:
Estados Unidos