Expression of PD-L1 Is Increased by Methionine Restriction Using Recombinant Methioninase in Human Colorectal Cancer Cells.
Cancer Genomics Proteomics
; 21(4): 395-398, 2024.
Article
in En
| MEDLINE
| ID: mdl-38944421
ABSTRACT
BACKGROUND/AIM:
It has been recently demonstrated that a methionine-restricted diet increases the response to immune checkpoint inhibitors (ICIs) via an increase in PD-L1 in a syngeneic mouse colorectal-cancer model. Our laboratory has developed recombinant methioninase (rMETase) to restrict methionine. The aim of the present study was to determine if rMETase can increase PD-L1 expression in a human colorectal cancer cell line in vitro. MATERIALS ANDMETHODS:
We evaluated the half-maximal inhibitory concentration (IC50) value of rMETase on HCT-116 human colorectal cancer cells. HCT-116 cells were treated with rMETase at the IC50 Western immunoblotting was used to compare PD-L1 expression in HCT-116 cells treated with and without rMETase.RESULTS:
The IC50 value of rMETase on HCT-116 was 0.79 U/ml. Methionine restriction using rMETase increased PD-L1 expression compared to the untreated control (p<0.05).CONCLUSION:
Methionine restriction with rMETase up-regulates PD-L1 expression in human colorectal cancer cells and the combination of rMETase and ICIs may have the potential to improve immunotherapy in human colorectal cancer.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carbon-Sulfur Lyases
/
Recombinant Proteins
/
Colorectal Neoplasms
/
B7-H1 Antigen
/
Methionine
Limits:
Humans
Language:
En
Journal:
Cancer Genomics Proteomics
Journal subject:
BIOQUIMICA
/
GENETICA MEDICA
/
NEOPLASIAS
Year:
2024
Document type:
Article
Affiliation country:
Estados Unidos