IKIP downregulates THBS1/FAK signaling to suppress migration and invasion by glioblastoma cells.
Oncol Res
; 32(7): 1173-1184, 2024.
Article
in En
| MEDLINE
| ID: mdl-38948026
ABSTRACT
Background:
Inhibitor of NF-κB kinase-interacting protein (IKIP) is known to promote proliferation of glioblastoma (GBM) cells, but how it affects migration and invasion by those cells is unclear.Methods:
We compared levels of IKIP between glioma tissues and normal brain tissue in clinical samples and public databases. We examined the effects of IKIP overexpression and knockdown on the migration and invasion of GBM using transwell and wound healing assays, and we compared the transcriptomes under these different conditions to identify the molecular mechanisms involved.Results:
Based on data from our clinical samples and from public databases, IKIP was overexpressed in GBM tumors, and its expression level correlated inversely with survival. IKIP overexpression in GBM cells inhibited migration and invasion in transwell and wound healing assays, whereas IKIP knockdown exerted the opposite effects. IKIP overexpression in GBM cells that were injected into mouse brain promoted tumor growth but inhibited tumor invasion of surrounding tissue. The effects of IKIP were associated with downregulation of THBS1 mRNA and concomitant inhibition of THBS1/FAK signaling.Conclusions:
IKIP inhibits THBS1/FAK signaling to suppress migration and invasion of GBM cells.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain Neoplasms
/
Signal Transduction
/
Cell Movement
/
Glioblastoma
/
Thrombospondin 1
/
Focal Adhesion Kinase 1
/
Neoplasm Invasiveness
Limits:
Animals
/
Humans
Language:
En
Journal:
Oncol Res
Journal subject:
NEOPLASIAS
Year:
2024
Document type:
Article
Affiliation country:
China