Differential diagnostic value of simultaneous detection of CD69 and HLA-DR on host T and NK cells in QFT-TB assay for identifying active tuberculosis.
Tuberculosis (Edinb)
; 148: 102537, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-38954896
ABSTRACT
BACKGROUND:
Interferon-gamma release assay (IGRA) for tuberculosis (TB) remains limited in its ability to discriminate between active TB (ATB) and latent TB infection (LTBI). Activation markers on host T and NK cells are currently considered to be promising markers in the diagnosis of ATB.METHODS:
This prospective observational study enrolled 213 participants and the participants were divided into ATB, LTBI, other lung-related diseases (ORD), and health control (HC) groups. CD69 and HLA-DR on T and NK cells were detected in QFT-TB assay, and a composite scoring system (TB-Flow) was created for the diagnosis of ATB.RESULTS:
The expression of activation markers (CD69 and HLA-DR) were significantly increased in ATB. HLA-DR on NK cells, CD69 on T cells, and QFT-TB in the differential diagnosis of ATB and HC were all of good diagnostic value (AUC>0.90). In addition, the TB-Flow greatly improved the efficiency of differential diagnosis between ATB and LTBI (AUC=0.90, 95%CI 0.84-0.96), with sensitivity and specificity of 79.17 % (95%CI 64.60%-89.04 %) and 88.68 % (95%CI 76.28%-95.31 %).CONCLUSIONS:
CD69 and HLA-DR on host T and NK cells are promising markers in distinguishing different TB infection status. Our blood-based TB-Flow scoring system can distinguish ATB from LTBI with good diagnostic efficacy.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Killer Cells, Natural
/
Antigens, Differentiation, T-Lymphocyte
/
HLA-DR Antigens
/
Antigens, CD
/
Lectins, C-Type
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Interferon-gamma Release Tests
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Tuberculosis (Edinb)
Year:
2024
Document type:
Article
Country of publication:
Reino Unido