Dose-response study on the transfer of pyrrolizidine alkaloids from a tansy ragwort extract (Jacobaea vulgaris Gaertn.) to bovine milk.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
; 41(9): 1144-1157, 2024 Sep.
Article
in En
| MEDLINE
| ID: mdl-38959287
ABSTRACT
Ragworts like tansy ragwort (J. vulgaris Gaertn., syn. Senecio jacobaea L.) contain hepatotoxic and cancerogenic pyrrolizidine alkaloids (PA) and their corresponding pyrrolizidine alkaloid N-oxides (PANO). Due to increasing spread of ragworts (Jacobaea spp.) PA/PANO may pose a health risk to animals and humans consuming contaminated feed and food. Therefore, the aim of the present study was to investigate the transfer of individual PA/PANO originating from a well-defined PA/PANO extract into the milk of dairy cows. For this objective, 16 German Holstein cows were assigned to four treatment groups (n = 4) in a 28-day dose-response study. Administration into the reticulorumen was performed daily by gavage after the morning milking. Three groups received different amounts of the J. vulgaris extract resulting in a PA/PANO exposure of 0.47, 0.95, or 1.91 mg PA/PANO/kg body weight/day, respectively. Furthermore, a control group received molasses to account for the sugar content of the used PA/PANO extract. While the composition of the PA/PANO extract was more diverse, the PA/PANO pattern in milk was dominated by the PA in their free base form. It was shown that mainly PA considered stable in the rumen environment were transferred into the milk. The main compounds in milk were jacoline (74.3 ± 2.4% of the PA/PANO sum), jaconine (11.2 ± 1.3%), and jacobine (7.2 ± 0.6%) with concentrations up to 29.7, 4.65 µg/l, or in the highest exposed group, 3.44 µg/l. There was no dose-dependent effect on the total PA/PANO transfer rate into the milk. The average transfer rate was 0.064 ± 0.005% of the administered content.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pyrrolizidine Alkaloids
/
Senecio
/
Plant Extracts
/
Milk
/
Dose-Response Relationship, Drug
Limits:
Animals
Language:
En
Journal:
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
/
Food addit. contam. , Part A, Chem. anal. control expo. risk assess. (Print)
/
Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment (Online)
Journal subject:
CIENCIAS DA NUTRICAO
Year:
2024
Document type:
Article
Affiliation country:
Alemania
Country of publication:
Reino Unido