Nanomechanics of CCN1-Mediated Staphylococcus aureus Phagocytosis.
Nano Lett
; 24(28): 8567-8574, 2024 Jul 17.
Article
in En
| MEDLINE
| ID: mdl-38959438
ABSTRACT
Phagocytosis is an essential mechanism of the human immune system where pathogens are eliminated by immune cells. The CCN1 protein plays an important role in the phagocytosis of Staphylococcus aureus by favoring the bridging of the αVß3 integrin to the bacterial peptidoglycan (PG), through mechanical forces that remain unknown. Here, we employ single-molecule experiments to unravel the nanomechanics of the PG-CCN1-αVß3 ternary complex. While CCN1 binds αVß3 integrins with moderate force (â¼60 pN), much higher binding strengths (up to â¼800 pN) are observed between CCN1 and PG. Notably, the strength of both CCN1-αVß3 and CCN1-PG bonds is dramatically enhanced by tensile loading, favoring a model in which mechanical stress induces the exposure of cryptic integrin binding sites in CCN1 and multivalent binding between CCN1 lectin sites and monosaccharides along the PG glycan chains.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phagocytosis
/
Staphylococcus aureus
/
Integrin alphaVbeta3
/
Cysteine-Rich Protein 61
Limits:
Humans
Language:
En
Journal:
Nano Lett
/
Nano lett
/
Nano letters
Year:
2024
Document type:
Article
Affiliation country:
Bélgica
Country of publication:
Estados Unidos