Development of a hepatic cryoinjury model to study liver regeneration.
Development
; 151(15)2024 Aug 01.
Article
in En
| MEDLINE
| ID: mdl-38975841
ABSTRACT
The liver is a remarkable organ that can regenerate in response to injury. Depending on the extent of injury, the liver can undergo compensatory hyperplasia or fibrosis. Despite decades of research, the molecular mechanisms underlying these processes are poorly understood. Here, we developed a new model to study liver regeneration based on cryoinjury. To visualise liver regeneration at cellular resolution, we adapted the CUBIC tissue-clearing approach. Hepatic cryoinjury induced a localised necrotic and apoptotic lesion characterised by inflammation and infiltration of innate immune cells. After this initial phase, we observed fibrosis, which resolved as regeneration re-established homeostasis in 30â
days. Importantly, this approach enables the comparison of healthy and injured parenchyma within an individual animal, providing unique advantages to previous models. In summary, the hepatic cryoinjury model provides a fast and reproducible method for studying the cellular and molecular pathways underpinning fibrosis and liver regeneration.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Liver
/
Liver Regeneration
Limits:
Animals
Language:
En
Journal:
Development
/
Development (Camb.)
/
Development (Cambridge)
Journal subject:
BIOLOGIA
/
EMBRIOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Australia
Country of publication:
Reino Unido