Functional Analysis of Seed Dormancy Genes by Biolistic Transient Gene Expression in Immature Embryos of Wheat.
Methods Mol Biol
; 2830: 131-136, 2024.
Article
in En
| MEDLINE
| ID: mdl-38977574
ABSTRACT
Seed dormancy genes typically suppress germination and cell division. Therefore, overexpressing these genes can negatively affect tissue culture, interfering with the generation of transgenic plants and thus hampering the analysis of gene function. Transient expression in target cells is a useful approach for studying the function of seed dormancy genes. Here, we describe a protocol for transiently expressing genes related to seed dormancy in the scutellum of immature wheat (Triticum aestivum) embryos to analyze their effects on germination.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Seeds
/
Triticum
/
Germination
/
Gene Expression Regulation, Plant
/
Plant Dormancy
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2024
Document type:
Article
Affiliation country:
Japón