Prinsepia utilis Royle polysaccharides promote skin barrier repair through the Claudin family.
Skin Res Technol
; 30(7): e13848, 2024 Jul.
Article
in En
| MEDLINE
| ID: mdl-38978226
ABSTRACT
BACKGROUND:
Plant polysaccharides have various biological activities. However, few studies have been conducted on the skin barrier of Prinsepia utilis Royle polysaccharide extract (PURP). MATERIALS ANDMETHODS:
The proportions of polysaccharides, monosaccharides and proteins were determined by extracting polysaccharides from fruit meal using water. The healing rate was measured by cell scratch assays. SDS-damaged reconstructed human epidermal models, an acetone-ether-induced mouse model and an IL-4-induced cellular inflammation model were used to detect the effects of polysaccharides on the phenotype, HA, TEWL, and TEER, with further characterizations performed using QRT-PCR, Western blotting, immunofluorescence (IF) assays.RESULTS:
PURP contained 35.73% polysaccharides and 11.1% proteins. PURP promoted cell migration and increased skin thickness in a reconstructed human epidermis model. The TEWL significantly decreased, and the HA content significantly increased. PURP significantly increased the TEER and decreased the permeability of the SDS-damaged reconstructed human epidermis model. Claudin-3, Claudin-4, and Claudin-5 were significantly upregulated. IF and Western blot analysis revealed that the Claudin-4 level significantly increased after treatment with PURP. Claudin-1, Claudin-3, Claudin-4, and Claudin-5 gene expression and IF and immunohistochemical staining were significantly increased in mice treated with acetone-ether. PURP promoted the expression of Claudin-1, Claudin-3, Claudin-4, and Claudin-5 after treatment with 100 ng/mL IL-4. PURP also downregulated the expression of NO, IL6, TNFα and NFκB in Raw 264.7 cells and in a mouse model.CONCLUSION:
We hypothesize that PURP may repair the skin barrier by promoting the expression of the claudin family and can assist in skin therapy.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides
/
Plant Extracts
/
Claudins
Limits:
Animals
/
Humans
Language:
En
Journal:
Skin Res Technol
Journal subject:
DERMATOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
Reino Unido