The P2X7 Receptor is a Master Regulator of Microparticle and Mitochondria Exchange in Mouse Microglia.
Function (Oxf)
; 5(4)2024 Jul 11.
Article
in En
| MEDLINE
| ID: mdl-38984997
ABSTRACT
Microparticles (MPs) are secreted by all cells, where they play a key role in intercellular communication, differentiation, inflammation, and cell energy transfer. P2X7 receptor (P2X7R) activation by extracellular ATP (eATP) causes a large MP release and affects their contents in a cell-specific fashion. We investigated MP release and functional impact in microglial cells from P2X7R-WT or P2X7R-KO mice, as well as mouse microglial cell lines characterized for high (N13-P2X7RHigh) or low (N13-P2X7RLow) P2X7R expression. P2X7R stimulation promoted release of a mixed MP population enriched with naked mitochondria. Released mitochondria were taken up and incorporated into the mitochondrial network of the recipient cells in a P2X7R-dependent fashion. NLRP3 and the P2X7R itself were also delivered to the recipient cells. Microparticle transfer increased the energy level of the recipient cells and conferred a pro-inflammatory phenotype. These data show that the P2X7R is a master regulator of intercellular organelle and MP trafficking in immune cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microglia
/
Mice, Knockout
/
Cell-Derived Microparticles
/
Receptors, Purinergic P2X7
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Mitochondria
Limits:
Animals
Language:
En
Journal:
Function (Oxf)
Year:
2024
Document type:
Article
Affiliation country:
Italia