RNA thermometers are widespread upstream of ABC transporter genes in bacteria.
J Biol Chem
; 300(8): 107547, 2024 Aug.
Article
in En
| MEDLINE
| ID: mdl-38992441
ABSTRACT
RNA thermometers are temperature-sensing non-coding RNAs that regulate the expression of downstream genes. A well-characterized RNA thermometer motif discovered in bacteria is the ROSE-like element (repression of heat shock gene expression). ATP-binding cassette (ABC) transporters are a superfamily of transmembrane proteins that harness ATP hydrolysis to facilitate the export and import of substrates across cellular membranes. Through structure-guided bioinformatics, we discovered that ROSE-like RNA thermometers are widespread upstream of ABC transporter genes in bacteria. X-ray crystallography, biochemistry, and cellular assays indicate that these RNA thermometers are functional regulatory elements. This study expands the known biological role of RNA thermometers to these key membrane transporters.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
ATP-Binding Cassette Transporters
Language:
En
Journal:
J Biol Chem
Year:
2024
Document type:
Article
Affiliation country:
Estados Unidos
Country of publication:
Estados Unidos